What is replica plating used for?

The purpose of replica plating is to be able to compare the master plate and any secondary plates, typically to screen for a desired phenotype.

What is the replica plate technique used for isolation of?

nutritional mutants
Replica-Plating Technique: Replica-plating technique is often used to isolate nutritional mutants (auxotrophs) as well as various other type of mutants, e.g., antibiotic resistant mutants.

Which technique is used for isolation of mutants?

1. Replica Plating Technique: Lederberg and Lederberg (1952) have given replica plating technique. This technique is used to detect auxotrophic mutants which differentiates between mutants and wild type strains on the basis of ability to grow in the absence of an amino acid.

What is the purpose of the replica plating method quizlet?

Why do we use replica plating? Replica plating may be used to test the ability of a variety of bacteria to grow on a number of different types of media.

How do you replica plate a yeast?

To replica plate, invert the master plate, the one with the yeast on it, over the velveteen on the holder and press the plate firmly against the sterile fabric surface. Then slowly peel the plate off, leaving a replica or print of the cell pattern on the velveteen.

How do you isolate auxotrophic mutants?

All the auxotrophic mutants isolated were obtained by the filtration enrichment method. The results in Table 2 show the variety of amino acid-deficient mutants that were isolated. Only one vitamin-deficient mutant was found using the filtration enrichment method. These results are in agreement with those of Bos et al.

What is the idea behind replica plating quizlet?

Replica plating is a transfer technique that may be used to produce multiple copies of a particular colonial arrangement on a number of agar plates.

Which mutants are isolated by gradient plate method?

This experiment concerns with the isolation of streptomycin resistant mutants using a prototrophic Escherichia coli by the use of a simple gradient technique where streptomycin at the rate of 100ug/ml is added in the nutrient agar medium.

How would you isolate analogue resistant mutants?

Isolation of the analogue resistant mutant The cells were washed twice with sterile TM buffer and then were spread on the surface of the agar medium containing the varying concentration of analogue higher than the MIC.

What plating method is used to grow multiple types of bacterial colonies?

The streak-plate procedure is designed to isolate pure cultures of bacteria, or colonies, from mixed populations by simple mechanical separation. Single colonies are comprised of millions of cells growing in a cluster on or within an agar plate (Figure 1). A colony, unlike a single cell, is visible to the naked eye.

Why velvet cloth is used in replica plating?

Using the threads of velvet or chamios leather which act as inoculating needles, the mutants can be replicated and isolated.

Why is velvet used in replica plating?

Why are cells plated on a nutritionally complete plate in addition to nutrient deficient plates when looking for a mutant?

At least one plate should also be nutritionally complete to ensure that cells are being properly transferred between the plates. The other plates lack specific nutrients, allowing the researcher to discover various auxotrophic mutants unable to produce specific nutrients.

Why gradient plate technique is used?

Gradient Plate Technique: An excellent way to determine the ability of organisms to produce mutants that are resistant to antibiotic is to grow them on a gradient plate of a particular antibiotic.

What are analogue resistant mutants?

The isolation of analog resistant mutants. 2. The isolation of revertants. An analog is a compound that is very similar in structure to another compound. Analogs of amino acids and nucleotides are frequently growth inhibitory, and their inhibitory properties may be due to a number of possible mechanisms.

What type of plate is used to isolate colonies select all that apply?

STREAK PLATE METHOD OF ISOLATION. The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. It provides a simple and rapid method of diluting the sample by mechanical means.

How is replica plating useful for negative selection?

Replica plating is especially useful for negative selection. For example, if one wanted to select colonies that were sensitive to ampicillin, the primary plate could be replica plated on a secondary Amp+ agar plate .

Why is replica plating used to isolate an auxotrophic mutant from a prototrophic parent?

Why is replica plating used to isolate an auxotrophic mutant from a prototrophic parent? Because there is no medium on which the auxotroph will grow and the prototroph will not. Therefore, direct selection is not possible.