How can DCPIP be used as an indicator?
DCPIP can be used to measure the rate of photosynthesis. It is part of the Hill reagents family. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction.
What is DCPIP protocol?
2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.
Is DCPIP a molecule?
2,6-dichlorophenolindophenol, ( DCPIP ) is a blue chemical compound used as a redox dye….
| DCPIP | |
|---|---|
| Chemical formula | C12H7NCl2O2 |
| Molecular mass | 268.1 g mol−1 |
| Density | x.xxx g cm−3 |
| Melting point | xx.x °C |
How do you calculate DCPIP concentration?
The concentration is measured by calculating the volume of solution* used to reduce the blue colour of D.C.P.I.P to colourless. By using a burette, the mixture of lemon juice, distilled water and glacial acetic acid is titrated into the D.C.P.I.P solution. The volume is noted when the blue colour change to colourless.
How does DPIP become colorless?
Oxidized (blue) DPIP becomes colorless when it is reduced that is, when it receives electrons. A diagram of the photosynthetic light reaction shows that water molecules are the source of these electrons.
Does DPIP reduced or oxidize?
DPIP Lab-Does DPIP reduced or oxidized? DPIP gains electrons, thus it was reduced. This replaces NADP in the light reaction since DPIP has a higher affinity for electrons than does NADP.
What is the function of the DPIP in this experiment?
The function of the DPIP in this experiment is to act as the electron acceptor, replacing the usual NADP found in plants. When the light shines on the active chloroplasts, the electrons are excited, which causes them to jump to a higher energy level thus reducing the DPIP.
How does diuron affect photosynthesis?
Diuron inhibits photosynthesis by preventing oxygen production and blocking the electron transfer of photosystem II of photosynthetic microorganisms.
How do you measure the amount of DCPIP in a solution?
The amount of DCPIP reduced can be found by measuring the solution’s light transmittance with a spectrophotometer. DCPIP can also be used as an indicator for Vitamin C.
Does the colour change when the DCPIP is exposed to light?
Tube 1 (leaf extract + DCPIP) colour changes until it is the same colour as tube 4 (leaf extract + distilled water). Tube 2 (isolation medium + DCPIP) no colour change. This shows that the DCPIP does not decolourise when exposed to light. Tube 3 (leaf extract + DCPIP in the dark) no colour change.
How do you measure complex II enzyme catalyzed reduction of DCPIP?
If DCPIP is used in the assay, the complex II enzyme-catalyzed reduction of DCPIP is measured by following the decrease in absorbance due to the oxidation of DCPIP at 600 nm (extinction coefficient for DCPIP 19.1 mM − 1 cm − 1 ). 25 mM potassium phosphate buffer, 5 mM MgCl 2, pH 7.2
What is the colour of DCPIP in tube 5?
Tube 5 (supernatant + DCPIP) no colour change if the supernatant is clear; if it is slightly green there may be some decolouring. The results should indicate that the light-dependent reactions of photosynthesis are restricted to the chloroplasts that have been extracted.