How do you make a 5% phenol solution?
Phenol is moderately soluble in water – about 8 g of phenol will dissolve in 100 mL water. So, take 5 gm phenol crystals and add water to 100 mL.
What is phenol solution used for?
Phenol is used primarily in the production of phenolic resins and in the manufacture of nylon and other synthetic fibers. It is also used in slimicides (chemicals that kill bacteria and fungi in slimes), as a disinfectant and antiseptic, and in medicinal preparations such as mouthwash and sore throat lozenges.
Is phenol A hazardous material?
Overview. The major hazard of phenol is its ability to penetrate the skin rapidly, causing severe burns. Toxic and even fatal amounts of phenol can be absorbed through relatively small areas of skin. Phenol may be fatal if swallowed, inhaled or absorbed through the skin.
How do you make a 1% phenol?
Take 100g phenol bottle to fume hood, open it, and pour in ~ 100 ml 50 mM TrisCl pH 8. Close lid tightly and shake gently. Leave to stand for an hour or two until the phenol liquifies and the phases are separated. Remove the supernatant with a pipette (dispose into the ‘chlorinated solvent waste’ container).
Why phenol is used as disinfectant?
Phenolic compounds used as antiseptics or disinfectants include pure phenol and substitution products with halogens and alkyl groups. They act to denature and coagulate proteins and are general protoplasmic poisons. Phenol (carbolic acid) is one of the oldest antiseptic agents.
How do you use phenol liquid?
Phenol liquid is often used in molecular biology with trichloromethane and chloroform to separate RNA, DNA, or proteins, and isolate them in the pure form. This process is known as liquid-liquid extraction. It’s done by adding an equal amount of phenol and chloroform to a solution of cells or tissues.
How much phenol is toxic?
Phenol is a general protoplasmic poison (denatured protein) with corrosive local effects. Phenol derivates are less toxic than pure phenol. The lethal dose is between 3 to 30 g, but may be as little as 1 g. Phenol is well absorbed by inhalation, dermal application, and ingestion.
Is phenol lethal?
Is phenol toxic to humans?
Phenol is highly irritating to the skin, eyes, and mucous membranes in humans after acute (short-term) inhalation or dermal exposures. Phenol is considered to be quite toxic to humans via oral exposure.
What are the hazards of phenol?
Here a few tips to avoid exposure: Be careful at work. Being exposed to phenol in industrial facilities Trusted Source may increase your risk of heart disease. Don’t eat anything that might contain phenol. Consuming phenol in its pure form can damage your esophagus, stomach, intestines, and other digestive organs. Don’t put it on your skin. Don’t inhale it. Don’t drink it.
What is the structural formula for phenol?
Phenol, also known under an older name of carbolic acid, is a toxic, colorless crystalline solid with a distinctive sweet tarry odor. Its chemical formula is C6H5OH and its structure is that of a hydroxyl group (-OH) bonded to a phenyl ring; it is thus an aromatic compound.
Is phenol a hazard?
The major hazard of phenol is its ability to penetrate the skin rapidly, causing severe burns. Toxic and even fatal amounts of phenol can be absorbed through relatively small areas of skin.
What is the formula of phenol?
Phenol, also known as phenolic acid, is an aromatic organic compound with the molecular formula C6H5OH. It is a white crystalline solid that is volatile. The molecule consists of a phenyl group (−C6H5) bonded to a hydroxy group (−OH). It is mildly acidic and requires careful handling due to its propensity to cause chemical burns.