How fundus autofluorescence works?
Unlike fluorescein angiography, FAF does not require the injection of a fluorescein dye in order to image the retina, but rather utilizes the fluorescent properties of lipofuscin within the retinal pigment epithelium (RPE) to create an image.
What is fundus autofluorescence imaging?
Fundus autofluorescence (FAF) is a non-invasive retinal imaging modality used in clinical practice to provide a density map of lipofuscin, the predominant ocular fluorophore, in the retinal pigment epithelium.
What is an autofluorescence test?
Fundus autofluorescence is a non-invasive diagnostic test that involves taking digital photographs of the back of the eye without a contrast dye.
What causes autofluorescence?
Autofluorescence is the tissue-endogenous fluorescence caused by several different fluorophores. These include collagen and elastin as components of the connective tissue, tryptophan as a component of most proteins, and nicotinamide adenine dinucleotide (NAD), a coenzyme found in all living cells.
What is bacterial autofluorescence?
Autofluorescence is the spontaneous fluorescence of materials without added dyes. Many people are responsible for this work but principal credit goes to Cindy Maddera, who just received her Master’s in my lab. Also contributing were Shelly Theiss, and Amy Huseby.
How do I get rid of autofluorescence?
Use fluorophores that emit in a wavelength further from the autofluorescence compounds in your sample. Typically, far-red wavelength fluorophores such as CoralLite 647 are best for this. Commercially available reagents such as TrueVIEW (VectorLabs), have been shown to reduce autofluorescence from multiple causes.
Does collagen have autofluorescence?
Collagen is highly autofluorescent. The best way to get rid of it is to subtract fluorescence images prior to adding the antibody from images take after staining with the antibody.
Do drusen cause vision loss?
Drusen do not cause total blindness, but can result in central vision loss. Central vision allows us to focus on details straight ahead. People with more soft and larger drusen, are at higher risk of experiencing this type of vision loss in the future than people with fewer and smaller drusen.
What is fundus autofluorescence imaging (FAF)?
New technology and research has spurred a renewed interest in fundus autofluorescence imaging (FAF) as a diagnostic technique for documenting the presence of fluorophores in the human eye. Fluorophores are chemical structures that possess fluorescent properties when exposed to light of an appropriate wavelength.
What is autofluorescence signal AF (AF)?
The Autofluorescence Signal AF was first reported in the 1970s, when autofluorescent signal of optic nerve head drusen was noted prior to the injection of fluorescein dye.[25] Additional FAF was noted soon thereafter with retinal astrocytic hamartoma and Best disease (BD).[26]
What is the role of autofluorescence in multifocal choroiditis?
Fundus Autofluorescence in Multifocal Choroiditis and Panuveitis Patients with MCP have much more widespread involvement of the RPE than would be suspected by other means of imaging. Autofluorescence photography supplies information about inflammatory damage and secondary CNV in a noninvasive manner.
What is the difference between autofluorescence and fafaf?
FAF is used to record fluorescence that may occur naturally in the eye or accumulate as a byproduct of a disease process. The term “autofluorescence” is used to distinguish this type of fluorescence from that which occurs from administration…