Is pET 28 an expression vector?
The pET-28b(+) expression vector used as the template for plasmid modification was obtained from Novagen. The YEpADH2p plasmid used for colony selection in S.
What is a pET28a vector?
The pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.
What is the copy number of pet28a?
(Plasmid #25630)
Is pET28a a high copy plasmid?
I work with the pET28a vector as well and it is a low copy number plasmid.
Is T7 promoter leaky?
However, the T7 promoter is known for background (“leaky”) expression, which can be a drawback when recombinant proteins are toxic to the host cell. Therefore, our vectors contain the lac operator (lacO) sequences immediately downstream from the promoter to reduce leaky expression.
Where is the single polyclonal site of the pet28a vector?
The single polyclonal site of the pET28a vector is seen above the circular plasmid map. Note: The vector sequence is encoded according to the coding rules of the pBR322 plasmid, so the T7 protein expression region is reversed on the plasmid map.
What is the his•tag of the pet-28a-c(+) vector?
TB074 12/98 The pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map.
How is the expression of pet28a induced from host cells?
The expression was induced by T7 RNA polymerase from host cells. The target gene was cloned into plasmid vector and controlled by strong phage transcription and translation signals. The single polyclonal site of the pET28a vector is seen above the circular plasmid map.