What is LC-MS PPT?
INTRODUCTION • Principle: • LC/MS is a technique that combines physical separation capabilities of liquid chromatography with mass analysis capability of Mass spectrometry. • It is a method that combines separation power of HPLC with detection power of Mass spectrometry.
Which is used as interface in LC-MS techniques?
API serves as both the ionization source and the interface in a LCMS system. Three API techniques are described here, namely the electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI).
Why is it called LC-MS MS?
LC-MS instruments are basically HPLC units with a mass spectrometry detector attached to it whereas LC-MS/MS is HPLC with two mass spectrometry detectors. The LC in LC-MS stands for liquid chromatography.
How HPLC is different from LC?
The difference between traditional LC and HPLC is that the solvent in LC travels by the force of gravity. In the application of HPLC, the solvent travels under high pressure obtained by means of a pump to overcome the pressure drop in the packed column, which reduces the time of separation.
What is the difference between HPLC and LC-MS?
HPLC is a separation technique that employs mainly to separate, identify and determine each component in the sample of a complex mixture. LC-MS is a hybrid system in which a UV or PDA detector of HPLC replaced with a mass spectrometer. HPLC results are less accurate than LCMS.
What is an LC instrument?
Pharmaceutical Liquid Chromatography Mass Spectrometer (LC/MS Instrumentation) LC/MS identifies proteins, detects metabolites, determines the molecular weight of drugs, verifies molecular structures and detects for impurities and degradants.
What is the difference between HPLC and LC?
Which detector is used in LC-MS?
An MS chromatogram for a single mass often produces an interference-free signal that offers high precision and low minimum detection limits. Using both a UV detector and a mass selective detector is more effective than using either one alone.
What is LC testing?
Liquid Chromatography Mass Spectrometry (LC-MS) is a method or technique used commonly for drug analysis, food analysis, and environmental testing. Liquid Chromatography Mass Spectrometry combines the physical separation and detection sensitivity capabilities of both platforms.
What is the principle of LCMS?
INTRODUCTION • Principle: • LC/MS is a technique that combines physical separation capabilities of liquid chromatography with mass analysis capability of Mass spectrometry. • It is a method that combines separation power of HPLC with detection power of Mass spectrometry.
How does LC-MS/MS work?
The principle of LC-MS/MS is based on the fragmentation of charged ions and the detection of the resulting fragments.
• It is a method that combines separation power of HPLC with detection power of Mass spectrometry. • In LC-MS we remove the detector from the column of LC and fit the column to interface of MS. • In the most of the cases the interface used in LC-MS are ionization source. 3.
What is LC-MS in pharmacology?
61. LC-MS has proved to be an extremely sensitive and specific technique for the analysis of pharmaceuticals. It plays important role in the studies of drug metabolism, discovery of new drug candidates and the analysis, identification and characterisation of impurities and degradants in drug substances and products. VIGNAN PHARMACY COLLEGE 61