What is RI detector in hplc?
HPLC Refractive Index Detectors (HPLC RI Detectors) are used with high-pressure liquid chromatographs when detecting substances with limited or no UV absorption. These chemical components included alcohols, sugars, fatty acids, polymers and carbohydrates. RI detectors are considered universal detectors.
What is the difference between UV detector and RI detector?
RI detector has lower sensitivity compared to UV detector, and that’s the main reason why RI is not as commonly used as UV. A UV detector cannot be used with solvent which has UV absorbance. Sometimes the organic solvent used for GPC analysis absorbs UV, and thus UV detector cannot be used.
How does RI detector work?
Principle: The RI detectors measure a bulk property of the mobile phase leaving the column: its ability to refract to bend light (i.e., its refractive index). This property changes as the composition of the mobile phase changes, such as when solutes from the column.
What is Dad detector?
Diode-Array Detection (DAD) or Photodiode-Array Detection (PDA) is an analytical technique that can be used to determine the purity of an analyte or related impurity peak eluting during an HPLC separation. The diode array detector uses the same principles of operation as a variable wavelength detector (VWD).
Which detector is called as universal detector?
In the current detector market, the mass spectrometer has been accepted as the most universal detector for gas chromatography with capabilities of both trace quantitative and definitive qualitative information.
Are there different kinds of refractometers?
There are four main types of refractometers: traditional handheld refractometers, digital handheld refractometers, laboratory or Abbe refractometers (named for the instrument’s inventor and based on Ernst Abbe’s original design of the ‘critical angle’) and inline process refractometers.
What is Dad HPLC?
Figure 1: Example of cannabinoids measured by (U)HPLC with Diode-Array detection. The photo diode array (PDA), also known as the diode array detector (DAD) can measure the entire wavelength range in real time, which may provide other advantages.