What is 100 bp DNA ladder?

What is 100 bp DNA ladder?

100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation. 100 bp DNA Ladder is ideal for separation on 1–2% agarose gels.

Where can I learn more about the Invitrogen DNA ladder?

Learn more at thermofisher.com/ladderupgrade17. Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.

What is the DNA ladder used for?

The DNA Ladder is suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA Ladder contains 100bp -1.5 kb DNA fragments. The 500bp band (100 ng/5 μl) has doubled intensity than other bands to serve as reference band.

How much DNA ladder do I need to dilute in buffer?

We recommend loading 0.5 μg of 100 bp DNA Ladder diluted in sample buffer. All fragments have 4-base, 5´ overhangs that can be end labeled using T4 Polynucleotide Kinase (#M0201) or filled-in using DNA Polymerase I, Klenow Fragment (#M0210) (1).

When was the ladder upgraded to the latest model?

This product was upgraded on September 15, 2017. Learn more at thermofisher.com/ladderupgrade17.

Why is the ladder designed with a uniform intensity?

The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

Does neneb offer a quick-load DNA ladder with purple dye?

NEB also offers a Quick-Load version of this ladder with purple dye. Recommended gel percentage range: 1.2-3% 100 bp DNA Ladder visualized by ethidium bromide staining on a 1.3% TAE agarose gel.