How stable is TCEP in solution?
Considerations for use of TCEP: TCEP is stable in aqueous, acidic, and basic solutions. When TCEP is dissolved directly in water, the resulting pH is approximately 2.5. TCEP is not particularly stable in phosphate buffers, especially at neutral or alkaline pH.
Is TCEP stable at room temperature?
Stable at room temperature. Stable in aqueous, acidic & basic solutions. We can ensure stability 72 hours without significant degradation or oxidation.
What is TCEP concentration?
TCEP (tris(2-carboxyethyl)phosphine) is a potent reducing agent and due to its odorless characteristic is a substitute for DTT and beta- mercaptoethanol use. Supplied at 0.5M that is 100X concentration. Recommended usage is at a final concentration of 5 mM or Use at 10µL/mL.
How do you inactivate TCEP?
In general >99% inactivation is achieved after 5–10 minutes at 25–37°C. To avoid reactivation, maintain a low concentration of reducing agent, 0.1–0.5 mM DTT or TCEP, or use prolonged incubation times with 10–20 mM TCEP upon inactivation.
Is TCEP volatile?
TCEP hydrochloride is an odorless (non-volatile) reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol.
Does TCEP go bad?
TCEP retains stability when stored at an acidic or alkaline pH; avoid storage at pH 7.0-8.0. Also avoid exposure to high concentrations of phosphate ions (>150 mM). Stock solutions are stable for up to 3 months at -20°C.
Does TCEP reaction with iodoacetamide?
Unlike DTT and other commonly used reductants, the TCEP does not compete with the alkylation reagent iodoacetamide. The kit is supplied with a proprietary Reductant Buffer necessary for an efficient reduction of disulfide bonds while minimizing re-oxidation of the competing thiol pairs in protein samples.
Does TCEP react with maleimide?
TCEP can keep the cysteines from forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily with the maleimide. However, TCEP has been reported to react with maleimide under certain conditions. TCEP is also used in the tissue homogenization process for RNA isolation.
How do you store TCEP?
TCEP retains stability when stored at an acidic or alkaline pH; avoid storage at pH 7.0-8.0. Also avoid exposure to high concentrations of phosphate ions (>150 mM). Stock solutions are stable for up to 3 months at -20°C. Getz, E.B., et al.
Is TCEP stable in HCl?
TCEP is stable in aqueous, acidic, and basic solutions. Studies indicate that no change in concentration of TCEP occurs after 24 hour incubation at room temperature in 100 mM HCl, 100 mM NaOH, and most of 50 mM buffer system.
What is the solubility of TCEP in methanol?
TCEP has only minimal solubility in organic solvents, including methanol and ethanol. TCEP is stable in aqueous, acidic, and basic solutions. Studies indicate that no change in concentration of TCEP occurs after 24 hour incubation at room temperature in 100 mM HCl, 100 mM NaOH, and most of 50 mM buffer system.
Can tctcep reduce protein disulfide bonds?
TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive cross-linking reactions. The ability and virtues of trialkylphosphine compounds to reduce protein disulfide bonds have been known for many years.1,2
Does concentration of TCEP change after 24 hours of incubation?
Studies indicate that no change in concentration of TCEP occurs after 24 hour incubation at room temperature in 100 mM HCl, 100 mM NaOH, and most of 50 mM buffer system.