Why are HepG2 cells used?

Why are HepG2 cells used?

Hepatic cells are important in the drug development process since the liver is the main detoxifying organ in the human body and mainly responsible for drug metabolism and drug-drug interactions. Hence, HepG2 cells are widely used for drug development and toxicity testing.

How do you Trypspize HepG2?

To trypsinize HepG2 cells, you need to wash the cells monolayer with pre-warmed PBS solution (1x) twice. Then, use pre-warmed trypsin containing 0.02% EDTA for 7min. Try not to hit the flask because they will aggregate.

What is a HepG2 cell?

HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions.

What are HuH-7.5 cells?

HuH-7 is a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male in 1982. The line was established by Nakabayshi, H. and Sato, J. HuH-7 is an immortal cell line of epithelial-like tumorigenic cells.

Are HepG2 cells adherent?

HepG2 cells have adherent properties and grow as monolayers in small aggregates. HepG2 can be grown successfully at a large scale and stimulated with human growth hormone. They are also capable of secreting many plasma proteins, such as transferrin, fibrinogen, plasminogen and albumin.

What is a cell line in cell culture?

Cell line. (Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised.

What is the culture culture protocol for HepG2 cells?

HepG2 cells were cultured in 5% CO2at 37°C in growth medium [RPMI 1640 (Gibco, Austria) supplemented with 10% (v/v) fetal calf serum (FBS, Gibco/BRL), 10 μg/mL streptomycin and 100 mg/mL penicillin (Sigma, CA, USA)]. Cells were subcultured every 5 – 7 days.

What is the morphology of HepG2 cells?

HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions. In this chapter, freezing, thawing, and subculturing procedures for HepG2 cells are described.

What is the pathophysiology of Hep G2?

Hep G2 is an immortal cell line which was derived from the liver tissue of a 15-year-old American adolescent boy of European ancestry with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Can HepG2 cells be culture in DMEM+10% FCS?

I guess HepG2 is a cancer cell line so it could be OK to culture in lower serum medium. But I used to culture then in DMEM+10% FCS and they are fine. Don’t worry. HepG2 cells are tough!