How does contamination affect cell culture?
Consequences of cell culture contamination Contaminants can affect all cell characteristics (e.g. growth, metabolism, and morphology) and contribute to unreliable or erroneous experimental results.
How do you test for contamination in cell culture?
Look for signs of turbidity or cloudiness of the media. Take 1ml of your culture/potentially contaminated media/new cell/cells fresh from storage and add it to 14 ml of media in a tube. Incubate and examine by eye and under your microscope at 400X magnification.
What is contamination in cell culture?
Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, endotoxins, plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma, as well as cross contamination by other cell lines.
What is an example of bacterial contamination?
Microbial contamination, also known as biological, is the most common cause of food poisoning. It is basically the existence of harmful pathogens in food, like microorganisms, bacteria, viruses, mould, fungi, and toxins. An example of this would include the bacteria and toxins found in spoiled meat.
What is bacterial contamination?
Bacterial Contamination. They are typically a few micrometers in diameters, and can have a variety of shapes, ranging from spheres to rods and spirals. Because of their ubiquity, size, and fast growth rates, bacteria, along with yeasts and molds, are the most commonly encountered biological contaminants in cell culture.
What is cell culture contamination?
Cell Culture Contamination. Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, endotoxins , plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma , as well as cross contamination by other cell lines.
What organelles are found in bacteria?
Bacteria cells do have organelles, although generally these are fewer in number and less complicated than those found in plants and animals. Most commonly, bacteria contain ribosomes. These organelles are composed of RNA-rich granules located within the cytoplasm and are the site for protein synthesis.
What is cell line contamination?
There are two principal ways a cell line can become contaminated: cell cultures are often exchanged between research groups; if, during handling, a sample gets contaminated and then passed on, subsequent exchanges of cells will lead to the contaminating population being established, although parts of the supposed cell line are still genuine.