What does Enterotube II test for?
The Enterotube II is a compartmental plastic tube containing 12 media to test for: acid and gas production from glucose, lysine decarboxylase, ornithine decarbox- ylase, H2S, indole, acetoin (VP test), phenylalanine de- aminase, urease, citrate utilization, and acid production from adonitol, lactose, arabinose.
How many biochemical tests can the Enterotube II multitest system carry out?
Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification of members of the family Enterobacteriaceae, was tested and compared with the PathoTec test system in the identification of gram-negative bacilli isolated from clinical specimens.
How is Enterotube used to identify microorganisms?
The enclosed inoculating wire allows inoculation of all compartments in one step from one or a few single colonies of your unknown microorganism. The resulting combination of enterotube reactions, together with other metabolism tests can help you to identify unknown organisms.
What type of bacteria does Enterotube identify?
BBL Enterotube II is a biochemical identification system and must not be used directly with clinical specimens. Use isolates from appropriate media (see Test Procedure). BBL Enterotube II may be used to identify aerobic, facultatively anaerobic Gram negative rods (Enterobacteriaceae) isolated from any specimen.
What is the purpose of using Enterotube?
The Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What are the advantages of EnteroPluri test?
Principle of the method: EnteroPluri-Test makes possible the identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria isolated from clinical and environmental samples. The identification is based on biochemical tests performed on culture media containing specific substrates.
What are the advantages of a Multitest system?
It was concluded that the Enterotube system provides a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What type of specimen is used to inoculate an Enterotube?
The wire is sterile and need not be flamed. Inoculate by touching the wire to a well isolated colony from a Petri plate. Inoculate the growth from MacConkey (MAC), Eosin Methylene Blue (EMB), Salmonella Shigella (SS) or Hektoen Enteric (HE) agars, or from nonselective blood agar media.
What are the advantages of using EnteroPluri test kits?
EnteroPluri-Test makes possible the identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria isolated from clinical and environmental samples. The identification is based on biochemical tests performed on culture media containing specific substrates.
What is the name of the reagent utilized for indole testing?
Indole Spot Reagent: p-Dimethylaminocinnamaldehyde (DMACA) 10.0 gm.
What is the Enterotube technique?
What is the purpose of using the EnteroPluri II system?
What is Enterotube 2 used for?
The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase – negative, gram – negative, rod shaped bacteria of the family Enterobacteriaceae. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
How do I test my Enterotube II?
Place the Enterotube II into a test-tube rack tween them to allow for air circulation. with the GLU-GAS compartment pointing up- ward. SECOND PERIOD b.
How do the two Multitest systems work together?
The two multitest systems II has an inoculating wire that extends through all 12 were developed to work together. If an unknown gram- compartments of the entire tube.
What are the advantages and disadvantages of Enterotube?
The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony. It reduces the number of inoculations and the equipment needed to perform a series of biochemical tests for the identification.