What does Lipofectamine 3000 reagent do?
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable superior transfection performance and reproducible results. It delivers exceptional transfection efficiency into the widest range of difficult-to-transfect and common cell types (Figure 1) with improved cell viability.
What is the difference between Lipofectamine 2000 and 3000?
Lipofectamine 3000 reagent yields higher transfection efficiencies than Lipofectamine 2000 reagent when tested in a variety of cell lines.
What is Lipofectamine 3000?
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458)
Why is transfection used?
Transfection is a powerful analytical tool for study of gene function and regulation and protein function. The main purpose of transfection is to study the function of genes or gene products, by enhancing or inhibiting specific gene expression in cells, and to produce recombinant proteins in mammalian cells [3].
How is transfection used?
What is the best dose of lipofectamine 3000 reagent for transfection?
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75µL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.
What is the difference between Invitrogen Lipofectamine 2000 reagent and 3000 reagent?
Lipofectamine 3000 reagent delivered higher efficiency and protein expression than both Lipofectamine 2000 reagent and Invitrogen Lipofectamine LTX Reagent. Using the most biologically relevant cell lines provides the most relevant answers to your research questions.
What is Invitrogen™Lipofectamine 3000 transfection protocol?
Transfection protocol On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Invitrogen™Lipofectamine 3000 Transfection Reagent: Step Tube Complexation components Amount per well (24-well)
What is the difference between FuGene HD and Lipofectamine 3000?
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. Figure 2. The transfection protocol for Lipofectamine 3000 was developed to be easy to use while still ensuring optimum performance and reliability in a wide panel of cell lines.