What is MDCK MDR1?
MDCK-MDR1 cells originate from transfection of Madin Darby canine kidney (MDCK) cells with the MDR1 gene (ABCB1), the gene encoding for the efflux protein, P-glycoprotein (P-gp)2. MDCK-MDR1 helps to gain an understanding of the mechanism of drug efflux, and highlights early potential issues with drug permeability.
What is MDCK permeability?
The MDCK permeability screen (uni-directional) is performed to measure test compounds across a monolayer of MDCK cells. The cells seeded on 24- or 96-well plates to form a confluent monolayer are used to determine the efflux of the test compound when added to the apical side of the membrane.
Why are MDCK cells used?
MDCK cells are used for a wide variety of cell biology studies including cell polarity, cell-cell adhesions (termed adherens junctions), collective cell motility, as well as responses to growth factors.
What is MDCK assay?
MDCK-MDR1 permeability assay to investigate intestinal or CNS permeability. MDCK-MDR1 cells originate from transfection of Madin Darby canine kidney (MDCK) cells with the MDR1 gene (ABCB1), the gene encoding for the efflux protein, P-glycoprotein (P-gp).
Are MDCK cells tumorigenic?
A research report by Novartis showed that serum-free suspension-cultured MDCK cells are very tumorigenic, with as little as 10 cells being capable of forming tumors in nude mice.
Is P-glycoprotein the same as MDR1?
MDR1, more commonly referred to as P-gp or P-glycoprotein, is an efflux transporter that serves two major drug transport functions. Its role in modulating the tissue distribution of drugs is well established, particularly with respect to CNS exposure.
Where is MDCK from?
Derived by S. H. Madin and N. B. Darby from the kidney tissue of an adult female cocker spaniel, the MDCK cell line originated in September 1958. Since that time, the cells have been widely utilized to investigate the processing of beta-amyloid precursor protein, as well as the sorting of its proteolytic products.
How are MDCK cells harvested?
In addition to other cell types, including hepatocytes and endothelial cells, MDCK cell sheets noninvasively were harvested from PIPAAm-grafted dishes merely by reducing the temperature. The viability assay showed that the transferred cells were not damaged during the two-dimensional cell-sheet manipulation.
Where are A549 cells found?
lung tissue
A549 cells, as found in the lung tissue of their origin, are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across alveoli. If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask.
What is multidrug resistance mutation (MDR1)?
Multidrug Resistance Mutation (MDR1) What is the Multidrug Resistance Mutation (MDR1)? The word mutation refers to a change in an animal’s genetic code. The phrase ‘multidrug resistance mutation 1 (MDR1)’ refers to a specific mutation that can occur at a gene known as the MDR1 gene, also known as the ABCB1 gene.
Can dogs with MDR1 mutation take doxycycline?
Doxycycline (antibacterial drug)- While we know that doxycycline is pumped by P-glycoprotein (the protein encoded by the MDR1 gene), we have not documented any increased sensitivity to this drug in dogs with the MDR1 mutation compared to “normal” dogs.
What causes low recovery in mdck-mdr1 test?
If the recovery is very low, this may indicate problems with binding of the compound to the plate or accumulation of the compound in the cell monolayer. However, poor solubility is the most common reason for unexpected recoveries in the MDCK-MDR1 test system. What is the relationship between MDCK-MDR1 permeability and human intestinal absorption?
Is Imodium toxic to dogs with MDR1 mutation?
Loperamide (ImodiumTM; antidiarrheal agent)- At doses used to treat diarrhea, this drug will cause neurological toxicity in dogs with the MDR1 mutation. This drug should be avoided in all dogs with the MDR1 mutation.