What is MLPA technique?
MLPA (Multiplex Ligation-dependent Probe Amplification) is a recently introduced method, based on PCR principle, useful for the detection of different genetic abnormalities (aneuploidies, gene deletions/duplications, subtelomeric rearrangements, methylation status etc). The technique is simple, reliable and cheap.
What is Mlpa sequencing?
MLPA is a multiplex PCR assay that utilizes up to 40 probes, each specific for a different DNA sequence (mainly exons of a specific gene of interest), to evaluate the relative copy number of each DNA sequence.
What can Mlpa detect?
MS-MLPA is a multiplex PCR-based technique that can detect changes in gene copy number status, DNA methylation, and point mutations, simultaneously.
How do you make MLPA probes?
Design Parameters for Synthetic MLPA® Probes
- Length Criteria: Length of the hybridization sequence should be greater than 21 bases and the two hybridization sequence should be adjacent to each other.
- Amplicon Length: The designed probes should have a unique amplicon length.
What does Mlpa stand for?
MLPA
| Acronym | Definition |
|---|---|
| MLPA | Marine Life Protection Act (aka Marine Life Protection Act Initiative; also seen as MLPAI; California) |
| MLPA | Multiplex Ligation-dependent Probe Amplification |
| MLPA | Modified Link Pack Area |
| MLPA | Metropolitan Land Planning Act (Minnesota) |
What is MS MLPA?
Methylation-specific MLPA (MS-MLPA) is a variant of MLPA, which does not require sodium bisulfite conversion of unmethylated cytosine residues, but instead makes use of the methylation-sensitive endonuclease HhaI. MS-MLPA can be combined with copy number and point mutation detection in the same reaction.
What is the MLPA® technique?
Principle of the MLPA® technique: MLPA (Multiplex Ligation-dependent Probe Amplification) is a multiplex PCR method detecting copy numbers of up to 60 different genomic DNA sequences, which is able to distinguish sequences differing in only one nucleotide. MLPA reactions require only 50 ng of human chromosomal DNA.
What are the steps involved in MLPA reaction?
The MLPA reaction can be divided in five major steps (see figure 1): DNA denaturation/hybridisation: DNA is denatured and incubated overnight with a mixture of MLPA probes. MLPA probes consist of two separate oligonucleotides (LPO and RPO), each containing one of the PCR primer sequences.
What are MLPA probemixes?
MLPA probemixes have probes that target a specific genomic sequence. An MLPA probe consists of two parts: a left and a right probe oligonucleotide (LPO and RPO). The LPO and RPO contain PCR primer and DNA hybridisation sequences. An additional stuffer sequence in the RPO gives each probe a unique length.
What are the advantages of using MLPA?
MLPA offers a rapid means of scanning up to 40 loci for gene dosage, and is likely to be widely used in research and diagnostic settings. However, there are still some limitations.