What is pcDNA vector?
This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
What is pcDNA3 plasmid?
Plasmid: pcDNA3 Mammalian expression vector with the CMV promoter and a neomycin-resistance marker.
What is mammalian expression system?
Mammalian expression is the system of choice for studying the function of a particular protein in the most physiologically relevant environment, because it allows for the highest level of posttranslational processing and functional activity of the protein.
How do you transfect cells?
Transfection can be carried out using calcium phosphate (i.e. tricalcium phosphate), by electroporation, by cell squeezing, or by mixing a cationic lipid with the material to produce liposomes that fuse with the cell membrane and deposit their cargo inside.
What is the pcdna 3 1 vector used for?
This pcDNA™3.1 (+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. The pcDNA™3.1 Expression Vector Family
What is the storage temperature of the pcdna™3+ vector?
20 µg of this pcDNA™3.1 (+) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C.
What are the features of pcdna™3?
It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site. Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: For Research Use Only.
How many versions of pcdna are there?
The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression