How many times does HindIII cut lambda DNA?
Description: The HindIII digest of lambda DNA (cI857ind 1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1).
What is the HindIII lambda phage size marker?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
What are the sizes of the bands of the molecular weight marker lambda DNA restricted with HindIII?
The Lambda DNA is completely digested with HindIII to yield bands ranging from 0.125 kb to 23 kb, suitable for use as molecular weight standards for agarose gels. The marker is composed to 8 purified individual DNA fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564 and 125.
What is Lambda HindIII ladder?
The λ HindIII DNA Ladder is prepared by restriction digestion of phage Lambda DNA to completion with HindIII, followed by heat inactivation of the enzyme. The resulting 7 fragments range from 564 base pairs to 23.13 kilobases in size. The λ Hind DNA Ladder is supplied ready to use in loading buffer containing dye.
What is HindIII Lambda Digest?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How many bands are visible in the digested lambda DNA?
Lambda phage-DNA has 5 EcoRI restriction sites so after it should produces 6 bands but I can see only 4 out of 6 possible bands.
What does Hind and III refer to in the enzyme HindIII?
In the enzyme Hind III; ‘H in’ refers to Haemophilus influenzae, D refers to the strain of H. influenzae and III refers to the sequence In which this enzyme was discovered.
How many DNA fragments does HindIII digest of lambda DNA?
The HindIII digest of lambda DNA ( c I857 ind 1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How is DNA-HindIII digest visualized in lambda?
Lambda DNA-HindIII Digest visualized by ethidium bromide staining. 1.0% agarose gel. The full list of DNA fragments can be found in the chart below.
How to prepare lambda DNA restrictions for students?
Obtain enough crushed ice and ice containers (styrofoam cups) for each lab group. Fill a pan with water and adjust it to 55°C on a hot plate Fill a second pan with water and adjust it to 37°C on a hot plate while the students complete preparation of the restriction digests. Reconstitute the lambda DNA with sterile distilled water to 0.4 µg/µl.
How were the sizes of the lambda DNA bands determined?
The sizes were determined by comparison to a molecular ladder which has bands of known sizes when it is separated by electrophoresis at the same time as the digested λ DNA. The sites at which each of the 3 different enzymes will cut lambda DNA are shown in the maps Enzymes A, B and C below.