What are culture Collection Centres?
Culture collections are centres that provide authentic examples of organisms, often microorganisms or animal and/or plant cell cultures that can be grown or maintained in the laboratory. They normally have a public service role and often provide other biological resources and services.
What is the purpose of Subculturing?
Subculturing, also referred to as passaging cells, is the removal of the medium and transfer of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
Why is it necessary for the culture medium to be cooled to approximately 48 50o C before it is poured into petri plates?
Why is culture medium cooled to about 48° to 50°C before it is poured into Petri plates? The culture medium is cooled to minimize steam condensation and prevent excessive heat from drying out and damaging the specimen. Cooling it also decreases chances of personal injury.
What are stock cultures?
A culture of a microorganism maintained solely to keep it viable for subculture into fresh medium. …
What precautions should be taken during Subculturing?
The risk of cross contamination or misidentification increases when more than one strain is handled at a time. Additionally, when handling a microbial culture, work quickly and carefully in an environment that has minimal distractions. Avoid passaging and subculturing microbial strains too many times.
What are the three main types of microbiological culture?
These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.
What is stock culture?
[ stŏk ] n. A culture of a microorganism maintained solely to keep it viable for subculture into fresh medium.
How do you maintain stock culture?
Whatever the size of the laboratory ‘s stock culture collection, it is important that it is properly maintained. Traditionally this has been a matter of culturing isolates on agar slants of suitable media and then subculturing onto fresh slants at regular intervals.
How can stock culture be preserved?
A popular method for preserving microorganisms is through lyophilization, or freeze drying. This method is best for long term storage. An alternative method for long-term storage of microorganisms is cryopreservation. This process involves storing samples at sub-zero temperatures.
Why Culture Collection Centres?
Therefore, such important cultures must be deposited at National or International Culture Collection Centres. At these centres the cultures are properly maintained by the experts. These cultures are governed by stringent rules and regulations so that intellectual property rights of the depositors must be protected.
How do you maintain a large stock culture collection?
Whatever the size of the laboratory ’s stock culture collection, it is important that it is properly maintained. Traditionally this has been a matter of culturing isolates on agar slants of suitable media and then subculturing onto fresh slants at regular intervals.
Where can I find the culture collection and Research Center?
The Culture Collection and Research Center, Food Industry Research and Development Institute, P.O. Box 246, Hsinchu, 30099, Taiwan, R.O.C. Country code: TW. Index Herbariorum: 59. Active BRC: yes.
Where can I find the Canadian Phycological Culture Centre?
Canadian Phycological Culture Centre (formerly known as the University of Toronto Culture Collection of Algae and Cyanobacteria, UTCC), Department of Biology, University of Waterloo, 200 University Ave W., Waterloo, ON, CANADA N2L3G1. Country code: CA. WDCM number: 955.