What is REDTaq PCR?
REDTaq® ReadyMix™ is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel.
What are the 3 types of PCR?
Types of polymerase chain reaction-PCR Real-Time PCR (quantitative PCR or qPCR) Reverse-Transcriptase (RT-PCR) Multiplex PCR.
What does a primer do in PCR?
Primer. A primer is a short, single-stranded DNA sequence used in the polymerase chain reaction (PCR) technique. In the PCR method, a pair of primers is used to hybridize with the sample DNA and define the region of the DNA that will be amplified. Primers are also referred to as oligonucleotides.
What enzymes are used in PCR?
Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus).
What is RT PCR technique?
Real time RT–PCR is a nuclear-derived method for detecting the presence of specific genetic material in any pathogen, including a virus. This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process.
What is redtaq DNA polymerase?
storage temp. REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing.
What is redredtaq Readymix?
REDTaq® ReadyMix™ is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel.
What is Taq DNA polymerase used for?
This method for routine PCR amplification of DNA uses standard Taq DNA polymerase. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
How does the red dye affect the sequencing?
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.