What is tamoxifen used for in mice?

What is tamoxifen used for in mice?

Tamoxifen is widely used for the induction of genomic recombination in mice (double-)transgenic for floxed genes and Tamoxifen specific estrogen receptors (ER) coupled to Cre-recombinase (supplemental Table 1) (Hayashi and McMahon 2002).

What does Cre stand for?

CRE, which stands for carbapenem-resistant enterobacteriaceae, are strains of bacteria that are resistant to carbapenem, a class of antibiotics typically used as a last resort for treating severe infections when other antibiotics have failed.

What is UBC Cre?

Strain #007001 | Common Name: UBC-Cre-ERT2. When these UBC-Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in widespread cells/tissues. In Goodwin et al. 2019 Genome Res.

Is it safe to drink alcohol while taking tamoxifen?

Unlike aromatase inhibitors, which block estrogen production, tamoxifen interferes with a tumor’s ability to use estrogen. Alcoholcan increase tamoxifen’s side effects and reduce its effectiveness. Therefore, alcohol should be limited or avoided during tamoxifen treatment. Drinking alcohol while taking tamoxifen

What is the prognosis of nestin-creert2-traced cells after tamoxifen?

Remarkably, 4 days after tamoxifen many Nestin-creERT2-traced cells have mature neuronal morphology, and are positive for both neuronal nuclear antigen and the pluripotency marker Sox-2.

Are adult bone marrow nestin+ cells depleted by tamoxifen and diphtheria toxin?

In adult Nestin-creERT2iDTRmice, tamoxifen and diphtheria toxin treatment severely reduces the numbers of bone marrow Nestin+cells [9]. To date, however, it remains unclear whether both subsets of adult bone marrow Nestin+cells, Nestin-gfpbrightand Nestin-gfpdimcells, are equally depleted by this strategy or to what extent.

What is nestin-creert2?

Nestin-creERT2is an improved genetic approach to control Nestin lineage in a temporal manner [62], which is particularly relevant for studies on adult Nestin+cells (Fig. 2c). This transgenic mouse model expresses a fusion protein encoding crecoupled to the modified estrogen receptor ligand-binding domain (ERT2).