What is the base pair rule?
Base-pairing rule – the rule stating that in dna, cytosine pairs with guanine and adenine pairs with thymine add in rna, adenine pairs with uracil.
What is the length of one base pair?
Length measurements bp = base pair—one bp corresponds to approximately 3.4 Å (340 pm) of length along the strand, and to roughly 618 or 643 daltons for DNA and RNA respectively.
What is the width of each base pair?
The diameter of the B-DNA is ~20 Angstroms, and the distance between base pairs is ~3.4 Angstroms. The base pairing of opposite strands is stereochemically selective, Adenine always pairing with Thymine, and Guanine with Cytosine.
What is Chargaff’s rule 2 of base pairing?
Chargaff’s rule 2 is that the composition of DNA varied from one species to another. The base paring rules state that A always pairs with T and G always pairs with C.
Is cytosine A nucleoside?
The nucleoside of cytosine is cytidine. In Watson-Crick base pairing, it forms three hydrogen bonds with guanine.
How many base pairs does A DNA turn have?
10 1/2 base pairs
The variation of energy with the twist of the base pairs about the helix axis shows the straight DNA free in solution is most stable with about 10 1/2 base pairs per turn rather than 10 as observed in the solid state, whereas superhelical DNA in chromatin is most stable with about 10 base pairs per turn.
How many angstroms is A nucleotide?
Each subunit occupies 3.4 Angstrom units which is the same amount of space occupied by a single nucleotide unit.
How far apart are nucleotides?
Each turn of DNA is made up of 10.4 nucleotide pairs and the center-to-center distance between adjacent nucleotide pairs is 3.4 nm.
What are the base pairing rules for DNA?
What are the Base Pairing Rules for DNA. The base pairing rules for DNA are often called Chargaff’s rules of DNA base pairing. The two strands of DNA are held together by the hydrogen bonds formed between complementary nucleotides, forming the double-stranded molecule of DNA.
What is a base pair bp?
A base pair ( bp) is a fundamental unit of double-stranded nucleic acids consisting of two nucleobases bound to each other by hydrogen bonds. They form the building blocks of the DNA double helix and contribute to the folded structure of both DNA and RNA.
What is the average weight of a DNA base pair?
Average weight of a DNA basepair (sodium salt) = 650 daltons. 1.0 A 260 unit ds DNA = 50 µg/ml = 0.15 mM (in nucleotides) 1.0 A 260 unit ss DNA = 33 µg/ml = 0.10 mM (in nucleotides) 1.0 A 260 unit ss RNA = 40 µg/ml = 0.11 mM (in nucleotides) MW of a double-stranded DNA molecule = (# of base pairs) X (650 daltons/base pair)
What is Chargaff’s rule of DNA base pairing?
The base pairing rules of DNA is called the Chargaff’s rules of DNA base pairing. The four types of DNA nucleotides are adenine, guanine, cytosine, and thymine. Adenine and thymine are purines while cytosine and guanine are pyrimidines.