What is the Hodge test used for?
Modified Hodge test is a simple test which can be performed in the routine lab for detection of carbapenemases in isolates showing intermediate or sensitive zone diameter on disc diffusion.
How does the Modified Hodge test work?
Modified Hodge test (MHT) has been suggested as a screening test for carbapenemases. It is based on the inactivation of a carbapenem by carbapenemase-producing strains (test isolate) that enable a carbapenem -susceptible indicator strain (E.
Which is the most recommended test for detection of carbapenemase production?
Ertapenem has been recommended as the best screening agent for KPC detection because KPC producers are usually insusceptible to ertapenem, but may remain susceptible to other carbapenems.
What is modified carbapenem inactivation method?
Modified Carbapenem Inactivation Method In the mCIM, 1 μL loopfuls of Enterobacteriaceae or 10 μL loopfuls of P. aeruginosa or A. baumannii from blood agar plates was emulsified in 2 mL trypticase soy broth (TSB). A meropenem disk was then immersed in the suspension and incubated for a minimum of 4 h at 35°C.
How are carbapenemase producers detected?
Identification of carbapenemases is first based on conventional phenotypic tests including antimicrobial susceptibility testing, modified-Hodge test and carbapenemase-inhibitor culture tests. Second, molecular characterization of carbapenemase genes by PCR sequencing is essential.
Does meropenem lower seizure threshold?
For meropenem, doripenem, and ertapenem, the seizure rate for each agent is reported as less than 1%. However, as their use increases and expands into new patient populations, the rate of seizures with these agents may increase.
Is carbapenem broad spectrum?
Carbapenems (imipenem, meropenem, doripenem) possess broad-spectrum in vitro activity, which includes activity against many Gram-positive, Gram-negative and anaerobic bacteria; carbapenems lack activity against Enterococcus faecium, methicillin-resistant Staphylococcus aureus and Stenotrophomonas maltophilia.
What is CP cre?
Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae (CP-CRE) is a subset of all CRE. The subset is categorized by the production of carbapenemases (called CP-CRE), enzymes that break down carbapenems and related antimicrobials making them ineffective.
What is modified Hodge test (MHT)?
This post was most recently updated on May 13th, 2017. Modified Hodge Test (MHT) is a simple phenotypic test for detection of presence of carbapenemase enzyme in bacteria.
What is modified Hodge test for Enterobacter?
Modified Hodge Test• The Modified Hodge Test (MHT) detects carbapenemase production in isolates of Enterobacteriaceae. The most common carbapenemase found in Enterobacteriaceae is the Klebsiella pneumoniae carbapenemase (KPC).
Can modified Hodge test detect carbapenemases on disc diffusion?
Conclusion Modified Hodge test is a simple test which can be performed in the routine lab for detection of carbapenemases in isolates showing intermediate or sensitive zone diameter on disc diffusion. Keywords: Modified Hodge test, disc diffusion, carbapenemases
How do you do a modified Hodge test?
Procedure of Modified Hodge Test (MHT): Prepare a 0.5 McFarland dilution of the E.coli ATCC 25922 (indicator organism) in 5 ml of broth or saline. Dilute 1:10 by adding 0.5 ml of the 0.5 McFarland to 4.5 ml of MHB or saline. Streak a lawn of the 1:10 dilution of E.coli ATCC 25922 to a Mueller Hinton agar (MHA) plate and allow to dry 3–5 minutes.