What is Lyophilisation technique?
INTRODUCTION. Lyophilization or freeze drying is a process in which water is removed from a product after it is frozen and placed under a vacuum, allowing the ice to change directly from solid to vapor without passing through a liquid phase.
What is lyophilization slide share?
Lyophilization is performed at temperature and pressure conditions below the triple point, to enable sublimation of ice. The entire process is performed at low temperature and pressure by applying vacuum, hence is suited for drying of thermolabile compounds.
What is the basic principle of lyophilization?
The basic principle of lyophilization Lyophilization is a process whereby a product is dried by removing the water under low temperature and pressure.
What is Lyophilisation describe the instrumentation and application of Lyophilisation?
Lyophilization Overview Lyophilization is a drying process for preservation of sensitive pharmaceutical products. The three principal parts of the process—freezing, primary drying, and secondary drying—are conducted at different combinations of these temperatures and pressures.
How do you Lyophilize a sample?
The steps required to lyophilize a product in a batch process can be summarized as follows:
- Pretreatment / Formulation.
- Loading / Container (Bulk, Flask, Vials)
- Freezing (Thermal Treatment) at atmospheric pressure.
- Primary Drying (Sublimation) under vacuum.
- Secondary Drying (Desorption) under vacuum.
How do you Lyophilize bacteria?
Place the vials into a freeze-dryer chamber and apply the vacuum to the chamber according to the manufacturer’s instructions. Allow the culture time to completely lyophilize (dry out). This may range from a few hours to overnight depending on the volume of each sample and how many samples you have.
Why is lyophilization needed?
“If the bulk drug ingredients are not stable in liquid or frozen form, lyophilization is necessary. Lyophilization enables longer shelf life, often as long as two-five years and makes it much easier to transport the product.
What is the purpose of lyophilization?
Lyophilization is a water removal process typically used to preserve perishable materials, to extend shelf life or make the material more convenient for transport. Lyophilization works by freezing the material, then reducing the pressure and adding heat to allow the frozen water in the material to sublimate.
What are the steps in lyophilization?
A traditional lyophilization cycle consists of three steps; freezing, primary drying, and secondary drying [1]. During the freezing step, the liquid formulation is cooled until ice starts to nucleate, which is followed by ice growth.
What is the function of the condenser in Lyophilisation?
The lyophilizer’s cold condenser provides a surface for the water vapor to adhere and solidify. The condenser also protects the vacuum pump from the water vapor. About 95% of the water in the material is removed in this phase. Primary drying can be a slow process.
How do you Lyophilize something?